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Transfected 293T cells were washed with phosphate-buffered saline (PBS Fisherer) and solubilized in IP buffer (PBS, pH 7.4, 2% IGEPAL (Sigma), supplemented, prior to use, with Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts (Sigma)).
This analysis has thoroughly supplemented prior descriptions of the taxon [1], [21], [23] and highlighted several newly recognized morphological features of Acrocanthosaurus, many of which may suggest structural re-organization of the allosauroid skull that have accompanied trends of increased size through time.
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This extensive MPSS analysis confirms and supplements prior microarray floral expression studies and illustrates the utility of sequence survey-based expression analysis in functional genomics.
Full disclosure — this year we supplemented our prior years' YC data with publicly available data.
Fifteen µl of the resulting supernatants were mixed with 3 µl of 6× sample buffer (360 mM Tris-HCl [pH 6.7], 60% glycerol and 12% SDS), which was supplemented immediately prior to use with 150 µl of β-mercaptoethanol and 150 µl of a saturated bromophenol blue solution per 2.5 ml.
Online mass detection, supplemented with prior knowledge of past fragmentation events, guides precursor selection and fragmentation.
Focus group data therefore served as a form of requirements gathering (supplemented by prior observational studies) to inform intervention design.
Robinson noted that Simpson testified she was on seven supplements prior to meeting Xuereb in May, adding "there is not sufficient evidence to support the respondent's assertion regarding the source of the prohibited substance".
NCS = no concentrate supplement prior to abrupt weaning (n = 10).
CS = concentrate supplement prior to abrupt weaning (n = 10).
Hypokalemia was corrected using oral potassium supplements prior to AVS.
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