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Figure 4 shows the degradation of the mixed toxins supplemented onto the pretreated CS solids by A. resinae ZN1.
Figure 3 shows that the degradation performance of different single toxin supplemented onto the pretreated CS solids by A. resinae ZN1.
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Cells were seeded in supplemented KSFM onto collagen I-coated plastic dishes the day before transfection.
The cells were transferred onto supplemented minimal medium with or without 3 mM aminotriazole using a 6 × 8 pronged replica plater.
Conidial suspensions were made in sterile water plus 0.1% (w/v) Tween 80 and spread directly onto supplemented MM containing 200 mM CaCl2.
A polydimethylsiloxane (PDMS) well of 10 mm in diameter was used to seed ~10,000 M109 cells in 0.2 ml supplemented RPMI medium onto a culture dish for 24 hours.
If they were freeze dried, then the yeast was reconstituted with water and streaked onto supplemented yeast extract agar plates; (YES; 5% yeast extract, 3% glucose, 225 mg/L histidine, 225 mg/L adenine, 225 mg/L leucine, and 225 mg/L uracil), 2% agar Bacto agar (Becton Dickinson).
Indeed, as demonstrated in this study, adhesion and growth of osteoblasts onto HA supplemented BC were greater as compared to BC alone.
Nitrate (1.3 g NaNO3/kg0.75 of body weight (BW)), with and without β1-4 galacto-oligosaccharides or nisin was administered into the rumen through a fistula 30 min after the morning meal as a single dose, whereas, β1-4 galacto-oligosaccharides (20 g per day) or nisin (3 mg/kg0.75 of BW per day) was supplemented by sprinkling it onto the feed.
Serial dilutions were also spread plated onto TYG supplemented with tetracycline for enumeration of recipients and transconjugants and TYG containing eythromycin for enumeration of donors and transconjugants.
Overnight cultures of H. pylori X47 ΔureAB rpsL.erm grown on BHI based agar plates were subcultured onto plates supplemented with DENT (Oxoid) in lawns of approximately 2 cm in diameter.
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