Objective: We investigated how aging effects human chondrocyte yield, proliferation, post-expansion chondrogenic capacity, and response to specific growth factors supplemented during expansion.
Over the years, the current base game — there are four total now — is supplemented with expansion packs to provide new ways to play the game — and kill your Sims.
We supplemented the expansion medium of the ADSCs with 1000 IU/mL LIF and demonstrated that OCT4, Nanog, and Sox2 expression can be maintained more efficiently at the presence of LIF.
Our results indicate that a supplement, expansion, or replacement to/of the questionnaires are sought for, although it is recognized that the relatively small sample size in the current study may be, in part, responsible for the nondetectable differences in mood disorders.
First, we could expand HUD housing choice voucher program to accommodate more individuals under a new income level (slightly higher than low-income) and collaborate with different organizations or programs to supplement that expansion and allow individuals to find their own homes at a subsidized rate.
FGF-2-supplemented expansion potentiated subsequent chondrogenic differentiation as the FGF-2-expanded cells showed a much greater increase in type II collagen expression than non-FGF-2-expanded cells.
Basal growth media used for MSC expansion are supplemented with multiple nutrients and growth factors.
Confluent cells were then cultured in expansion medium supplemented with 50 mg/ml ascorbic acid for 3 weeks to facilitate cell sheet formation.
Ian de Sales de la Terriere, the present Laird is passionate about the place and has managed to run the estate on traditional lines for the last 40 years, a mix of sport and agriculture supplemented recently with an expansion into tourism.
Individual clones were isolated and further expanded on MEF cells in expansion medium supplemented with 20 µg/ml G418 in order to maintain transgene integration.
To address the possibility that M031+7 cells may manifest EGF-independent growth characteristics, we omitted EGF from the hNPC expansion media supplemented only with LIF.
