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They explained that each plebe first gets a ready-made outfit, which is supplemented as soon as possibly by form-fitting tailor-mades, both every day and dress.
Lentiviruses were propagated in 293T cells, maintained in DMEM supplemented as above.
Liposome-containing medium was replaced after 3 5 h by glutamate-free Neurobasal medium supplemented as above.
Thioglycolate elicited macrophages were obtained by peritoneal lavage 4 days after intraperitoneal injection with 3% thioglycolate and cultured in DMEM supplemented as above.
The amino acids were supplemented as needed.
Parallel controls were cultured in NBA (supplemented as described above).
MEFs were grown in DMEM supplemented as above.
Human melanoma cells UKRV-Mel-06a were grown in RPMI-1640 supplemented as above.
Cells were plated at 2 × 10/ml in specific medium supplemented as above.
Jurkat T lymphocytes were grown in RPMI-1640 supplemented as above.
The T24 cell line was grown in McCoy's medium supplemented as above.
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