Sentence examples for supplemented afterwards from inspiring English sources

You could even rub over the embroidery using a large coin - the outlines will not, of course, be very clearly defined upon the paper and will have to be gone over and carefully supplemented afterwards with a pencil.

Take a look at what it would cost to purchase retiree medical insurance, which you may need before you qualify for Medicare at age 65 and to supplement Medicare afterwards.

Tissue collection followed immediately with samples frozen in liquid nitrogen and was complete within 15 min. Animals were anesthetized with carbon dioxide and perfused transcardialy with physiological saline supplemented Heparin and afterwards with 4% paraformaldehyde and 0.1% glutaraldehyde in phosphate buffered saline (PBS).

The suspensions were afterwards supplemented with 3 µM MBP-mCherry and 1 µM ThioflavinT, a diagnostic dye for the presence of amyloid-like cross-β-structures.

All but five interviews were conducted by a single researcher (AG), informed by ongoing research on alcohol in Russia, taking contemporaneous notes supplemented by observations immediately afterwards.

Afterwards, the cultures were supplemented with streptomycin (50 µg/ml) for 2 hours to prevent de novo protein biosynthesis while allowing complete maturation of the fluorescent reporter protein.

Afterwards, new culture medium supplemented with polyclonal antibodies to PRV and guinea pig complement (Sigma) was added to prevent continuous infection pressure from the outer chamber to neurons in the inner chamber.

Afterwards the frozen sample was supplemented with 1 ml Trizol (Invitrogen) and homogenized two times (2 min, 1000 Hz) using the TissueLyser (Qiagen), followed by centrifugation (10 min, 20°C, 12,000 rpm).

Each interview was transcribed verbatim by the interviewer afterwards, and the transcript was supplemented with information from the field notes.

Afterwards, they were blocked with PBST supplemented with 2% (w/v) skim milk powder (2% MPBST) for 1 h.

Moreover, two replicate 24-well plates with standard growth medium were first exposed to IR (1 or 5 Gy) and afterwards exposed to standard growth medium supplemented with 10 ng ml−1 rhs TRAIL.