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The role of Si as a supplementation factor which is effective in decreasing Cd toxicity has been reported in cucumbers [ 98], maize [ 99– 101], rice [ 102– 104], Brassica chinensis [ 105], and peanuts [ 106].
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Four isoproteic (36% CP) and isolipidic (18% CL) diets were formulated in a 2 × 2 factorial design with FM/SBM levels and NSPase supplementation as factors.
However, the conditions seemed sub-optimal for oligodendroglial differentiation suggesting that oligodendroglia might require exogenous supplementation of factors.
When increased serum cobalamin concentrations are encountered clinically they are usually attributed to parenteral supplementation, dietary factors, or otherwise ignored.
In general, multiple myeloma cells, if removed from the bone marrow, often cannot survive without feeder cells or supplementation of factors to cell cultures, e.g. IL-6 in case of the INA-6 cell line [ 52].
Mice on a restricted diet have decreased circulating levels of leptin and tumour necrosis factor-α (TNFα), and supplementation of these factors to non-restriction levels suppresses PRH.
One reason for this could be the finding that tubule formation on 2-D surfaces was dependent on supplementation with different factors, including transforming growth factor (TGF -β1 [ 97].
After two weeks growth factor supplementation was removed, and the constructs were cultured for two additional weeks.
We finally cultured human mesenchymal stem cells on these scaffolds, in the absence of exogenous chondrogenic factor supplementation.
Here we describe the development of an anisotropic collagen-glycosaminoglycan (CG) scaffold and use of growth factor supplementation strategies to create a 3D platform for tendon tissue engineering.
Scaffolds with structural features similar to the extracellular matrix stimulate rapid osteogenic differentiation in favorable microenvironment and with growth factor supplementation.
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