Depending on your plan, you may want to also add room dividers or supplementary knee walls to your attic space.
Our previous data confirmed that rAAV 1/2-based vectors get trapped in the ventricle wall (Supplementary Fig. 1).
Co-staining using an antibody against phosphorylated histone H3 (pH3) suggests that CD31+/CD41+ cells proliferate in situ on the endocardial wall (Supplementary Fig. S4).
Spiral arteries from Lgals1 −/− females were characterized by increased wall/lumen ratio (Supplementary Figure 13a), wall thickness (Supplementary Figure 13b) and lumen diameter (Supplementary Figure 13c), which pointed to abnormal vessel function.
At E13.5, 83% of mutant embryos had superficial hemorrhages (Fig. 1D) and a thinner ventricular wall (supplementary material Fig. S3C,D).
The rate of ring contraction was similar to that observed in cells with an intact wall (supplementary material Fig. S4B).
There was no significant alteration of cell proliferation or apoptosis within the bronchial wall (supplementary material Fig. S2).
In sections of control siRNA electroporated slices, preplate formation occurred normally in both lateral and dorsolateral areas of the telencephalic wall (Supplementary Fig. 6 a,b ).
For identification of motor areas in the medial wall (supplementary motor area [SMA], pre-SMA, cingulate motor area [CMA]), we referred to the definitions of Roland and Zilles (1996) and Picard and Strick (2001).
In agreement with previous reports, the antibody intensely stained formations of cells in the shoulder regions of human carotid atherosclerotic plaques including the intima of capillaries within the arterial wall (Supplementary Fig. 2).
Lineage-tracing experiments with these Cre lines excluded a significant contribution from any of these cell types to coronary vessels within the ventricular-free wall (Supplementary information, Figure S10).
