Duplication sequence data including: (1) multiple-sequence alignment (MSA) of HSDs (fastas; labelled 1 through 19 corresponding to labels in Supplementary Dataset 2); (2) visualization of pairwise alignments from MSAs (Align_slider.pdf); and (3) contigs of HSD regions of CH17 BACs not included in reference genome (CH17_contigs.fasta).fasta
Following SDS PAGE, gel to membrane transfer and blocking, protein phosphorylation was assessed by Western blot using commercially available antibodies (catalogue numbers and dilutions supplied in Supplementary Table 1), and visualization of membranes was performed using ImageQuant TL v8.1 software (GE Healthcare).
For further details of segmentation and visualization, see supplementary material Methods.
The Sequenza software consists of two distinct parts: a python-based preprocessing tool, and an R package implementing the model fitting and visualization functions (supplementary Figure S1, available at Annals of Oncology online).
Supplementary Figures 1-9 include visualizations of how the index is calculated maps for sub-goal results, and a variety of outputs from correlation and sensitivity analyses.
For a better visualization, see the Supplementary Material for large frame-size movies of this experiment.
The reaction can be monitored by electrophoresis in a denaturing urea-polyacrylamide gel, followed by phosphorimaging visualization, and quantification (Supplementary Figure 1, available at Carcinogenesis Online).
See Supplementary Figure S2 for a visualization of the model structure, Supplementary Figures S3 and S4 for the averaged connectivity parameter estimates, and Table 1 for a numerical representation of the results.
Selecting any of the Structure-Tabs, by clicking on the PDB_IDs shown at the left side of the screen, presents the structure-related data, including the interactive pocket visualization module (see Supplementary Figure S6).
See Supplementary Information for three-dimensional models visualization of trackways VM1, VM2 and VM3.
See supplementary Information for three-dimensional models visualization of tracks VMX.1 and VMX.2.
