The model was used to explain the results of laboratory fatigue experiments, containing different LSP patch geometries, supplementary stress raising features and different specimen thickness.
This change, however, can be considered a supplementary stress factor.
Longitudinal gene neuroimaging studies, such as the IMAGEN study, aim to clarify the role of the HPA axis and supplementary stress systems in the development and maintenance of alcohol dependence.
A putative transcription regulatory gene similar to regulators involved in the xenobiotic response (xre) occurred upstream of genes, which were predicted to confer resistance to oxidative stress (supplementary table S2, Supplementary Material online).
In contrast, the expression of two genes located downstream of the aioBA operon, that is, cytC encoding the periplasmic cytochrome c552, which can serve as an electron acceptor to the arsenite oxidase (Santini et al. 2007) and moeA1 encoding a molybdenum cofactor biosynthesis gene, was not significantly affected under arsenite stress (supplementary table S6c, Supplementary Material online).
In addition to the canonical Ace1-regulated copper response common to all isolates, strains also responded to increasing copper toxicity by strain-specific upregulation of genes maintaining homeostasis under oxidative stress (supplementary fig. S9, Supplementary Material online).
To put the magnitude of this response in perspective, NIH3T3 cells responded to HS by up-regulating the mRNA levels of 186 and 791 genes and down-regulating 26 and 579 genes at 45 and 135 min, respectively, following application of stress (Supplementary file 1 and Supplementary file 2).
At the two highest copper concentrations, this variable upregulation extended to genes with roles in cell wall maintenance and resistance to pH stress (supplementary fig. S9 e g, Supplementary Material online).
On the contrary, we did not observe any effect of PKR inhibition on oxidative stress (Supplementary Figure S4) or microglial activation (Supplementary Figure S5).
The GO categories showed specific trends for many Pi-related genes in response to –P stress (Supplementary Fig. S5) and ++P stress (Supplementary Fig. S6).
Moreover, comparison of the fold change in RPKM values from control and treatment conditions with the normalized signal intensity clearly showed a similar pattern of expression profile obtained by mRNA-Seq and microarray-based analysis in all treatments of –P and ++P stress (Supplementary Table S3).
