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Supplementary feeding through specific AFS (i.e., supplied with food adequate for this species such as lamb legs), the most important management action applied for the conservation of this species, may have also been significant in this recuperation.
Moreover, the heterologous expression of ScASNase1 was obtained with high purity (see supplementary Fig. 1) through one chromatographic step with specific activity of 196.2 U/mg for L-Asn (Fig. 1A).
Cities with the largest populations for specific time periods are also highlighted in Supplementary Tables 2 through 8, a comparison with UN urban population data for the year AD 2000 is included in Supplementary Table 8.
Materials will consist of Robert Merges and John Duffy, Patent Law and Policy (3rd ed. 2002) and a set of supplementary readings available through the course homepage.
Fixation is with Ilizarov external fixation and supplementary Kirschner wires through the medial malleolus (d).
Supplementary requirements of the specific local structural situations were taken into account.
Supplementary Figure 6 Antigen-specific tTregs acquire specific pMHCII in vivo.
Supplementary Figure 2 Antigen-specific tTreg cells perform antigen-specific suppression in vivo.
Supplementary Figure 7 Antigen-specific iTreg cell treatment decreases DC function specifically.
Additional file 1 Specifics of case-study outbreaks, supplementary methods (Radiation Model of Mobility Fluxes, Surveillance Network Topologies, and Integrated Network Resilience) and supplementary Figures.
See Supplementary Data 6 for specific primers used.
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