In both Blau et al. (2011) and Blau and Kahn (forthcoming), we examine the behavior of married women separately, either as a main or a supplementary specification, and obtain similar results as for the full sample of women.
See Supplementary methods for specifications of the microscope and image acquisition.
The overall recovery was 65%, and endotoxin and host cell protein levels were within specifications (Supplementary Methods).
There is a 24-gene overlap between our 96-gene signature and that of Hoek et al., with 18 of the genes in common, including MITF, involved in melanin biosynthesis, pigmentation, development, and lineage specification (Supplementary Information S1), which reinforces the importance of these pathways in melanoma.
We found that concentrations lower than 50 ng/ml activin A did not markedly influence mesoderm specification (supplementary material Fig. S2).
Importantly, we also observed a significant increase in expression of the canonical Wnt/β-catenin inhibitor DKK1 in anterior-derived cardiogenic mesoderm, which suggests a more repressive state of Wnt/β-catenin signaling during cardiac specification (supplementary material Fig. S3B).
Pairwise comparison of RNA-Seq results between these cell populations at E15.5 and E18.5 revealed an increase in the expression of a number of genes important for β-cell specification (supplementary material Tables S4 and S5).
Furthermore, expression of RFP in the Tg ifabp:RFP) as200 transgenic line and neutral red uptake was unchanged, indicating normal intestinal bulb and mid intestinal specification (supplementary material Fig. S5B and S5C, respectively) (Flores et al., 2008; Oehlers et al., 2011a).
Detailed experimental analyses of the capacity of the SVC to satisfy the damping specifications of supplementary control are also presented via multiple contingencies.
The workflow process was carefully monitored with addition of positive and negative controls at each process step and individual samples were accepted for final analysis after passing quality control specifications (see Supplementary Text S1).
