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Supplementary to the database, supplementary software was developed including an NMR lab administration system.
Supplementary software includes programs that allow easy establishment of typical anisotropic tensor information for the numerical simulations as well as visualization of molecular structures with attached graphics of NMR-relevant information such as peptide planes, chemical shielding tensors, etc.
These are available as supplementary software and are described in more detail in Materials and Methods.
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Availability: Predictions are provided in supplementary tables; software and additional data are available from the authors by request.
The TEM images were created and analyzed by a supplementary Digital Micrograph software package.
Following Ruiz-Ruano et al. (2011), the samples were stained by Feulgen reaction and images obtained by optical microscopy were analyzed with the pyFIA software (supplementary fig. S5 and table S18, Supplementary Material online).
We plotted the values of free antibody against those of the peptide competitor using the BIAevaluation software (Supplementary Figure S7C).
ΔG of the nearest-neighbor interactions was calculated by Perl-based software (supplementary Data S1) using Kowalski's sliding-window approach [6].
Availability: The software, supplementary information and manual is available at http://bioputer.mimuw.edu.pl/software/bnf/.
For all other genes, the primers were designed using Primer 3 software (Supplementary Table 1).
The supplementary document and software are available at http://mlg.hit.edu.cn:8082/.
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