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We compared protein citrullination, PAD protein expression and PAD activity in extracts of normal brain and of normal-appearing white matter (NAWM) from MS patient brains using the slot blot method (supplementary material Table S1).

The results from select candidates obtained using the AFLP transcriptional profiling were verified using RT-qPCR and slot-blot RNA hybridization (supplementary material Fig. S2).

To establish the mapping parameters, 16 olive DNA sequences were selected, whose copy numbers per haploid genome were reported in the literature or were established by slot blot and hybridization experiments previously performed in our lab (supplementary material S1, Supplementary Material online).

The integrated transgene head-to-tail tandem copy number was estimated by slot blot from 1st generation offspring and ranged from 2 to approximately 200 (supplementary Table S1).

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