Confocal laser scanning microscopy analysis with FITC-labeled HIgG provided further evidence that HIgG specifically binds to the rSbpA-GG lattice on the emulsomes, and hence, rSbpA-GG coated emulsomes are functional in terms of specific IgG recognition (Supplementary data, Fig. 3).
This score also contained "Remember" responses, which showed a highly similar pattern [Fig. 2 d; main effect of action (F1,28 = 4.89, P = 0.035) and an interaction (F1,28 = 4.6, P = 0.041); Go Win vs. No-Go Win (t(28) = 2.79, P = 0.009); see Supplementary Materials for recognition memory].
Since all three residues required for catalytic activity of O-methyltransferase are conserved but those for S-adenosylmethionine binding are not completely conserved in As-dmtA (6 out of 8) (Zubieta et al., 2001), this insufficient complementation may be due to incomplete conservation of the latter or unidentified residues required for substrate recognition (supplementary material Fig. S3B).
The apparent differences in pause efficiencies can be explained by the various elongation properties of these RNAPs (such as hydrolysis efficiency, elongation rate and so on) as well as by variations in amino acids contacting the RNA DNA hybrid and potentially participating in the hybrid sequence recognition (Supplementary Table 2).
Box 1 Broad categories of codes (detailed codes given in online supplementary appendix 1) Awareness : Recognition of the need to understand leadership and management in general, its relevance to future work and the levels at which it applies.
Analyses of whole-brain contrasts were conducted on separate age groups so as to gauge the extent to which the different groups engaged the same or different regions in the recognition task (Supplementary Table 1 and Fig. 5).
A similar 'ionic latch' structure is found in AddB and site-directed mutagenesis experiments support the idea that it is unlocked in response to Chi recognition (see Supplementary Figure S7 for discussion).
Also consistent with previous studies, we found bilateral activation common to visual tactile matching, visual visual matching and tactile tactile matching in a region close to the activations in LOtv reported by Amedi et al. (2001) and Pietrini et al. (2004) during visual and tactile object recognition (see Supplementary Material).
By MALDI-TOF mass spectrometry analysis, the 18 immunoreactive proteins which exhibited different frequencies of recognition were identified (Supplementary Table 3).
The mass spectra of the purified protein suggested the incorporation of both 8 and phenylalanine at the S2 site (Supplementary Figure 2), indicating the recognition of 8 by PylRS N346A/C348A) is not significantly better than that of phenylalanine itself.
