"supplementary protocol" is a correct and usable phrase in written English.
It refers to a document or agreement that adds to or clarifies the terms of an existing protocol. Example: The two countries signed a supplementary protocol to the trade agreement, outlining specific tariffs for agricultural products.
The 2010 Nagoya Kuala Lumpur Supplementary Protocol calls on Parties to adopt a process to define rules governing liability and redress for damage from international movements.
A complete protocol for this method is available (Supplementary Protocol S1).
The resulting DNA was purified using Qiagen's QiaAmp DNA mini kit according to the manufacturer's supplementary protocol: "Purification of REPLI-g amplified DNA".
The products were biotinylated by ligating the Bio-T/B adapter (sequence in Supplementary protocol S1), MinElute column purified, made single-stranded and immobilized on streptavidin-coated magnetic beads.
The DNA concentration was measured by qPCR (Mx3005P Real Time PCR System, Stratagene, La Jolla, CA), the pool was further amplified for 15 cycles using the bridge primers (sequence in Supplementary protocol S1) and purified with the SPRI beads; the concentration of the 22 µl eluate was determined with the Bioanalyzer 2100 DNA 1000 chip (Agilent Santa Clara, CA).
50 µg of protein was applied to a denaturing polyacrylamide gel and subjected to electrophoresis (for details please see Supplementary protocol 1 and Supplementary protocol 2).
Countries that are signatories to the UNFCCC and its supplementary Kyoto Protocol are obliged to report changes in carbon pools.
Signatory countries to the United Nations Framework Convention on Climate Change (UNFCCC) and its supplementary Kyoto Protocol (KP) are obliged to report greenhouse gas emissions and removals.
The complete bioinformatics workflow is described in detail in the Supplementary Bioinformatics Protocol.
DNA preparation for other procedures was performed using the PureGene Genomic DNA Tissue Kit (Qiagen catalog number 158622), following a supplementary Qiagen protocol for nematodes.
Studies in which indels are particularly important, such as on human diseases [ 24, 25], might need to adapt the approach by deeper sequencing and allowing SAMtools to call indels (see online supplementary bioinformatics protocol) or by avoiding using 454 altogether.
