Furthermore, two genes, Saov 0148 and hysA, predicted to be involved in amino acid transport and polysaccharide degradation, respectively, had elevated d N/d S levels indicating diversifying selective pressure (supplementary table S4, Supplementary Material online).
In all cases analyzed, persistent changes of d N/d S (i.e., the variation is propagated to descending branches) provided a better fit than episodic changes (i.e., the variation is not propagated to descending branches), consistent with a long-term variation of the selection pressure (supplementary fig. S10, Supplementary Material online).
There were 75 genes which had multiple mutations indicating that these genes were under strong selection pressure (Supplementary Information Table S2).
As a direct result of the present initiative, an extensive set of data for computational tools validation is provided as Supplementary Materials, including pressure signals, wave height measurements and experimental videos.
Both E5.0 and E5.5 LembryosVestablishedos estasymmetricsymmetric Lefty1 expression during 24 h of culture, without the requirement of external biomechanical pressure (Supplementary information, Figure S3A-S3C).
Levels of IL-6 and IL-10 were significantly (P<0.05) positively correlated (Spearman's correlation) with SOFA and APACHE-II, and negatively correlated with mean arterial pressure (Supplementary Table 2- upper table).
As expected, examining rs2149954 in the International Consortium for Blood Pressure GWAS (24) showed a significant association of the minor allele with lower diastolic (P = 3.46 × 10−5) and systolic (P = 6.55 × 10−6) blood pressure (Supplementary Material, Table S9).
There were no clinically significant differences between treatment arms at baseline for mean serum levels of HbA1C and mean blood pressure (supplementary Table 7, available in an on online appendix).
