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Supplementary Figure 1 Preparation of ChIL probes.
qPCR analysis confirmed the successful capture of single-cell nuclei during library preparation (Supplementary Figure 1a).
See Supplementary Document for preparation of microscopy slides and immunofluorescence assay.
This file contains Supplementary Figure 1, preparation of [3H]vercirnon and vercirnon.
For label-free quantification, proteins were filtered for cell surface location, based on the cell surface protein atlas29 and the human surfaceome (Omasits, U. et al., manuscript in preparation; Supplementary Table 2).
Finally, we note that for [mo] and [Sha] amyloids generated at 37 °C with rotation, on occasion, two or more structural polymorphs could be observed within the same macroscopic fibril preparation (Supplementary Fig. 8 for representative data) as opposed to the single, "pure" strains associated with the NMR spectra shown in Figs. 4 and 5.
Supplementary Methods 2. Preparation of chromosomes from regenerating fin tissue.
Western blot also confirmed that Pn was enriched in the crude mitochondrial preparation (Supplementary Fig S2B).
Various errors can occur at different stages of Illumina library preparation (Supplementary Fig. S1).
LN contributed to study concept and design, data interpretation, supplementary analyses, manuscript preparation and revising and critical revisions of the manuscript.
The gel portions containing APPL1 (80 90 kDa) and actin (37 50 kDa) were excised and prepared as previously described [ 28, 30], and as outlined in the electronic supplementary material (ESM, Preparation of samples).
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