The spindle angle relative to the cell substrate adhesion plane (Supplementary Figure S2C) for MGRN1 RING mutant-expressing cells (∼55 70%, depending on the construct) was >10°, whereas control spindles were usually parallel to the substratum.
2. Shedding of CD31 + endothelial membrane-derived MVs visualized on the sequence of single optical planes Supplementary material 3 (TIFF 12725 kb).
Systematic analysis was best achieved by analysing data in section sequence, using axial, sagittal and then coronal planes (supplementary material Figs S1 S3), rather than organ by organ.
A clearer visualisation of the internalised HuHMFG-1 IgG colocalising with the endosomal marker can be seen in the gallery of images, which shows the cells after 15 min (maximal colocalisation) sliced through the Z-plane (supplementary Figure 1).
The cost-effectiveness planes (Supplementary data, Appendix S3 available in Age and Ageing online) show that the home intervention has a high chance of being cost-saving regardless of the value of a QALY gain.
This application ensured that the posterior aspects of the medial and lateral femoral condyles and the greater trochanter contacted the X- Y plane (see Supplementary Material Video 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/459612) and that the distal aspects of the medial and lateral femoral condyles contacted the X - Zplane (Supplementary Material Video 2).
The Whiteside's line, posterior condylar line and the tibial cutting plane were supplementary to judge the femoral rotation.
Furthermore, all dissections were performed according to a dissection protocol initiating dissection away from any potentially valuable tissue and working towards the surgical plane (see Supplementary data).
Modules assembled as sets of genes are projected as supplementary information onto the plane spanned by the two principal components issued from MFA.
Then, the 200-μm slice was immunolabeled for NeuN, and a maximum intensity projection of confocal NeuN fluorescence image stacks was acquired from the upper 15 μm of the slice (measured perpendicular to the slice plane, see Supplementary Fig. 1 B ).
