The majority, 61%, of the Pchlide extracted from the Δ chlB strain was in the 8E form, with 39% being the non-reduced pigment (Supplementary Figure S2, A at http://www.biochemj.org/bj/462/bj4620433add.htm).htm
If the king or queen dies, it is replaced by several supplementary reproductives that are slightly pigmented and have either short wing pads (brachypterous) or none (apterous) and reduced compound eyes.
This revealed that OCRL is expressed in the retina and the retinal pigment epithelium (RPE) (Supplementary Material, Fig. S2E).
Subsequently, the size of the compromise in the bilayer fluctuated frequently before the bilayer finally ruptured at either 120 s (Fig 1D) or 345 s (Supplementary Fig S3), depending on pigment concentration.
Three, six, five, and ten PKS genes are clustered with other genes involved in the biosynthesis of pigments, mycotoxins, lovastatin, and alternapyrone, respectively (supplementary fig. S7, Supplementary Material online), indicating that H. minnesotensis might produce secondary metabolites with similar structures.
Starting at the age of 8 months, vacuolated epithelial cells showed in addition a multifocal accumulation of granular, yellow brown autofluorescent pigment reminiscent of lipofuscin (Supplementary Material, Fig. S3A) and an indicator for cellular aging (19, 20).
By 24 hpi, the wound area was usually heavily pigmented (Fig. 1D,G-I; supplementary material Movie 1).
Photosynthetic pigments were quantified colorimetrically, which confirmed the breakdown of chlorophyll a (supplementary fig. S1, Supplementary Material online).
Three days post-injection, we could detect Sticky-trap in the subretinal space, between the retinal pigment epithelium (RPE) and outer nuclear layers (Supplementary Fig S17).
The gut cells of these animals showed reduced pigment granule birefringence under Nomarski optical microscopy (Supplementary Figure 8), and the anterior bulb of the pharynx exhibited abnormal morphology, with a balloon-like appearance and enlarged diameter (Supplementary Figure 8).
Also, we did not detect any protein on pigment samples analyzed on Sypro Ruby-stained SDS PAGE (Supplementary Fig S8).
