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The probe was in level with the tops of the eggs (Supplementary Picture S2d) and secured with a toothpick.
This system registered the identity of a tagged bird on the nest every 5 s throughout the incubation period (for technical details, see legend in Supplementary Picture S2).
A thin antennae loop (ø 9cm) was fitted into the nest cup and connected to a datalogger approximately 0.5 m outside of the nest (Supplementary Picture S2a c).
Adults were marked with an aluminum US Geological Survey band, a unique combination of 4 color bands, and a green flag with embedded glass passive integrated tag (Biomark: 9.0mm × 2.1mm, 0.087g, 134.2kHz, ISO FDXB, http://www.biomark.com/; Supplementary Picture S1).
To determine whether females and males differed in egg incubation temperatures, in 14 nests, instead of adding an external temperature probe, one egg was replaced with a solid egg (from PVC-U, painted to resemble a sandpiper egg; Supplementary Picture S3) with a high-resolution MSR® temperature probe (0.2 °C accuracy) positioned just under the egg surface.
Two types of loggers were used: the MSR® 145 at the nests with a fake egg (recording interval 5 s; Supplementary Picture S2c and d) and the HOBO Pendant® Temperature Data Logger (0.47 °C accuracy, UA-002-64, Onset Computer Corporation, http://www.onsetcomp.com/) at all other nests (recording interval 1min).
Similar(54)
Eight core subtests (block design, similarities, digit span, coding, vocabulary, letter-number sequencing, matrix peasoning, and symbol search) and two supplementary subtests (picture completion, and information) were administered.
In line with the tabulations (Table 3), the regression models of the BMI interaction showed a different picture (Supplementary Table S2).
To calculate the ratio of the diameter of normal mitochondrial tubules to the diameter of dynamic tubules, 20 cells were imaged in each experiment of three, and a pair of tubules (one normal mitochondrial tubule and one dynamic tubule) was cropped from the same picture (Supplementary information, Figure S13).
In addition to previously described procedures, in this study, we protected control and treatment nests against avian predators using enclosures made of mesh wire (Supplementary 1: Section 3 Pictures, Picture S1).
We made an artificial brood-patch by embedding a heat-producing egg in polystyrene (Supplementary 1: Section 3, Picture S3) and placed it over each nest scrape in contact with the artificial egg (Supplementary 1: Section 3, Picture S4).
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