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This supplementary pattern is not a preserved protein pattern according to web-based SMART program.
A deletion of 3 amino acids (amino acids 17 to 19) and a supplementary pattern of 6 amino acids were found in exon 4 of Ampelopsis japonica NPR1.2.
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Both cell lines presented a GlcNAc concentration-dependent change of O-glycosylation pattern (Supplementary Figure 6).
As expected, Rapamycin delays WH to 72 hours showing again dose-dependent pattern (Supplementary Figure 3).
The presence of Rad18 did not significantly affect this pattern (Supplementary Figure S1A).
The cell lines within the same group show quite different copy number pattern (supplementary Figure 5).
Addition of Mms2 and Rad5 did not significantly change this pattern (Supplementary Figure 1B).
This is not due to loss of its kinase activity as the kinase inactive RIP1 K45M does not show this expression pattern (Supplementary Figure S2b).
These genes were then clustered using the Cluster 3.0 software (30) according to their expression pattern (supplementary Figure 3A is available at Carcinogenesis Online).
Within 1 3 h after TNF stimulation, the tubular mitochondrial network of mito-GFP disintegrated in a punctate pattern (Supplementary Figure S5).
This individual shows an overall increased SUMOylation by SUMO-2/3, specifically in the 'scatter' pattern (Supplementary Figure 1, panel II, C).
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