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An unidentified protein, present also in endoAΔ4 null mutants, served as a convenient supplementary loading control (Figure 4A, asterisk).
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Blots were stained with methylene blue to show equal DNA loading (Supplementary S6; [ 45]).
The calcein fluorescence, in the absence of Co2+, was similar in both control and depleted cells, indicating equivalent calcein loading (Supplementary Figure 7).
The differences in age and possibly bisphosphonate use indicate that subtrochanteric fractures may be more related to mechanical loading, Supplementary material 1 3: see www.actaorthop.org, identification number 6660.
Additionally, we quantified the fraction of Ago2-loaded siRNA in relation to total intracellular siRNA which followed a hyperbolic saturation curve with increasing siRNA dose, with <1% fraction bound at the IC50 of knockdown and saturating at ca 4% maximal loading (Supplementary Figure S2I, J and M).
Secondly, a supplementary model for load balancing is set up for a better load distribution solution on feeders and transformers at TSC loading.
The Combined Heat and Power (CHP) unit has both absorption and electric chiller for cooling loads, and a supplementary boiler for heating loads.
Where it is necessary to carry out-of-plane loads, supplementary simple structural surfaces (SSSs) are provided, for example as floor cross-members and parcel shelves.
Lesion masking across the nine brains under study yielded a range of whole-brain lesion load (Supplementary Table 1), but with a common stereotypical distribution (Supplementary Fig. 2B).
These negative correlations were stronger in networks of high plaque load (Supplementary Fig. 3), suggesting a detrimental impact of amyloid-β pathology on intrinsic connectivity.
This strategy permits efficient and sensitive detection of mutations using DNA extracted from paraffin-embedded tissue, even in samples with relatively low tumour load (Supplementary Figure 1).
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