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However, by enhancing the training with supplementary labeled data from a related source domain, the model's ability to classify target instances can be improved.
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The aim of this study was to develop whole-mount tissue processing methods for 3D in situ visualization of the microvasculature that were also compatible with supplementary labeling for other structures of interest in the tissue microenvironment.
The mismatches that occurred in the stem (potentially influencing the secondary structure) and loop were then labeled (supplementary fig. S1, Supplementary Material online, shows a demonstration).
In addition, some, but not all, nuclei of cells located in the outer layers of the antennal lobes were labeled (supplementary material Fig. S5).
The gt_indel_2 and gt_indel_7 primer sets included an M13 site on the left primer to enable fluorescent labeling in a 3-primer reaction, while the gt_indel_9 left primer was directly fluorescently labeled (Supplementary file 1).
Each F2 population was fixed and prepared for FISH as previously described (Kowalinski et al., 2011) using custom Stellaris (Biosearch Technologies) probes for the Orsay virus RNA1 molecule (Supplementary file 1B) labeled with Quasar 670 Dye.
To minimize database bias, we chose randomly only one chromosome per species (1,215 chromosomes; see supplementary table S3, Supplementary Material online, chromosomes labeled red).
Distribution of the vesicular structures throughout the cell body and enrichment of fluorescent vesicles in cell extensions was confirmed by 3D reconstruction of labeled cells (Supplementary Fig. S1 and Supplementary Video S3).
BAF60 MOs were 3′ FITC labeled (Gene Tools) (supplementary material Table S1).
For each probe, regions between the primers listed in Supplementary Table S1 were labeled with P by random priming.
Primers (Supplementary Table 1) were labeled with [ γ-P] ATP following the polynucleotide kinase protocol (PNK; New England Biolabs).
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