Supplementary Figure 3 Isolation of interneurons migrating through the MZ and SVZ.
Supplementary Figure 1 Isolation and global methylation analysis of neuronal (NeuN+) and non-neuronal (NeuN−) nuclei from frozen brain tissues.
None of these known or putative H3K36me effector proteins showed similar phleomycin sensitivity as set2Δ in isolation (Supplementary Fig. 2d).
Replacement of the native sulfur donor with potassium hydrosulfide (KSH) as a sulfur donor surrogate26 led to increased 5-SH formation (Supplementary Fig. 23a), isolation of which allowed unambiguous structural characterization by 1H and 13C NMR (Supplementary Figs. 24 and 25).
Whole heart tissue was used in all cases for nucleic acid isolation (Supplementary material).
On the basis of searches in OMIM and literature, we selected 112 human genes known to cause MR, either as part of a known syndrome or in isolation (Supplementary Table 1).
Based on secondary clinical assessments following sample isolation (Supplementary Table 4), patients were retrospectively classified as 'Sepsis' (n = 37), or 'No Sepsis' (n = 35), consistent with current sepsis diagnostic criteria (see the Materials and Methods) (Lyle et al., 2014, Bone et al., 1992, Vincent et al., 2013, Levy et al., 2003).
Even though the selection of a pure SC population from muscle biopsies requires the use of multiple markers besides CD5621, the combined effect of our isolation protocol (Supplementary Fig. 1a) and culture conditions ensured the almost exclusive growth of satellite cell clones (SCCs, 57/58 tested colonies, Supplementary Table 1).
For LPL-T and adipocyte isolation, see Supplementary Materials online.
To this end, we performed PEx on DNA samples that were not treated with RNase A during the isolation procedure (Supplementary Material, Fig. S3).
For gene expression analysis, 300 ng of total RNA (for isolation, see Supplementary Methods, online only, available at www.exphem.org) were reverse transcribed using random hexamers.
