Therefore, we decided to restrict all model fits and tests to 32 out of the 36 data points where the repulsion effect is due to inhibitory interactions and not affected by yet unknown supplementary interactions (discarding surround angular deviations of ±10° and ±20°, see Fig. 5, grey data points).
The secondary structures, active site Cys, and GSH-binding residues appear to be preserved in most CC-type isoforms and many share the C-terminal ALWL motif crucial for TGA interaction (supplementary fig. S1, Supplementary Material online).
In OGD-treated astrocytes, 14-3-3 14-3-3 14-3-3trocytes via binding to p-Bad Ser112, but neither via p- β-catenin Ser 37 interaction (Supplementary Figure 10a) nor via Bax downregulation (Supplementary Figure 10b).
Both Dpb11-WG700,701AA anDpb11-W700A0A were unable to interact with Mec1 Ddc2 and Dpb11 YG735,736AA showed a reduced interaction (Supplementary Figure S2E).
We filtered the PIN1 network by removing self-interactions (giving rise to the PIN2 network, consisting of 8,280 proteins connected by 29,611 interactions; supplementary fig. S1, Supplementary Material online) and both self-interactions and interactions among proteins encoded by paralogous genes (PIN3, 8,189 proteins and 29,059 interactions; supplementary fig. S1, Supplementary Material online).
Salt concentrations of 0.2 M weakened the ClpX-FtsZ complexes and those of 0.5 M nearly abolished the interaction (Supplementary Fig. S4, panel A).
The methylation of the cytosine in the spacer region does not affect the protein DNA interaction (Supplementary Fig. 3).
Starting from the top level of the BIND schema, a 'BIND-interaction' record was converted to a PSI-MI interaction (Supplementary Figure S1).
The reciprocal experiment performed immunoprecipitating with anti-PATZ1 Ab and revealing with anti-p53 Ab confirmed the interaction (Supplementary Figure S1b).
To examine the subcellular localization of ADAM10/SAP97 complex formation, we used the previously characterized cell-penetrating Pro peptide, capable of interfering with the ADAM10/SAP97 interaction (Supplementary Figure 3C).
