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To visualize and clean up sequences, we used the freeware MEGA5; instructions on how to create a single-edited sequence per student using only freeware can be found in supplementary information material ESM 3 and ESM 6.
Details of the SNP selection process are provided in the supplementary information material (see Supporting information Appendix S1).
Detailed methods on inclusion of patients, processing of samples, EGFR and KRAS sequencing, oligonucleotide aCGH analysis, genomic PCR, fluorescent in situ hybridization studies, gene expression analysis and SNP array analysis are available in supplementary information (Material and Methods S1).
(for a detailed description of correlated uniquenesses across assessment conditions by age groups, see the supplementary information material, Table SM14).
(Supplementary information material describing these results—here, referring to Tables SM4 SM6 may be downloaded at http://www.geronto.uni-erlangen.de/pdfs/Langetal_BigFive_SIM.pdf).
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The accuracy of the lab results was verified with blanks and known standards (Supplementary Information, "Materials and Methods").
Additional information is provided in supplementary information, Materials and Methods.
Detailed protocol is shown in Supplementary Information (Materials and Methods).
Details of the technique are described in supplementary information, Materials and Methods.
The experimental procedure and the data processing are described in Supplementary Information Materials and Methods.
In order to identify THOC5-dependent transcripts, whole data were subjected to a multistep filtering procedure as described in Supplementary Information (Materials and Methods).
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