For the pairwise ortholog tables, between 0.2% and 1.3% of the annotations do not fully agree (supplementary file S1, Supplementary Material online), highlighting the difficult nature of reconciling gene functions of independently annotated genomes.
In addition, the e-beam stimulated quasi-melting process is compared with thermally stimulated melting processes in Supplementary Discussion 1 and Supplementary Table 2, highlighting the similarities and differences observed in the two processes.
ABC transporter genes were upregulated more frequently than GSTs, COEs or P450s in insecticide-resistant larvae (Supplementary Fig. 24), highlighting the potential role of ABC transporters in detoxification.
Interestingly, Desi3a-HA protein showed rapid degradation, within 10 min of flg22 treatment (Fig. 3h and Supplementary Fig. 22), highlighting a strong correlation between Desi3a turnover by flagellin and flagellin-induced FLS2 SUMOylation.
Notably, preventing ZKSCAN3 phosphorylation by mutating T153, a target site of JNK2/p38 MAPK upon PKCδ activation14, abolished PLX4720-induced ZKSCAN3 translocation (Supplementary Fig. 4c), highlighting a conserved regulation of ZKSCAN3 in BRAFV600E melanoma.
None of the other members of PRC1/PRC2 complexes we examined, including enzymes responsible for deposition of PcG-associated histone marks (Ring1a/1b, Ezh1/2), or members of the KAT and TrxG activating complexes, were direct targets of SCL (Fig. 6f, g, Supplementary Fig. 7), highlighting the unique relationship between SCL and RYBP/PCGF5.
Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation of these genes revealed that genes associated with membrane transport, transcription, and glycan biosynthesis and carbon metabolism were significantly enriched for expression in Hai7124 as compared to TM-1 fibers (Supplementary Fig. 21a), highlighting the species-specific role of these genes in fiber elongation.
The strong and sustained hypoxia resulted in the failure of muscle recovery, extensive tissue necrosis, and severe muscle mass loss (Fig. 4g, h, i, Supplementary Fig. 13) highlighting the importance of R-Ras-dependent endothelial lumenogenesis for the reperfusion of ischemic muscles.
All tested BiFC-positive TFs were found by co-ip (Supplementary file 3), highlighting that the S2 cell environment is appropriate for revealing interactions with tissue-specific TFs.
All cost components reported by studies within each cost category are summarised in online supplementary appendix 2, highlighting the considerable variability in obtaining and reporting cost aspects related to VI&B between all studies.
Other studies have also identified associations with protein levels, but not with genotype (Supplementary Table 1), highlighting the need to evaluate both MBL genotype and phenotype in infection and other immune conditions.
