Cells were counted before and after neutrophil enrichment and only neutrophil enriched samples (>90%) were used for subsequent DNA extraction (Supplementary Table 1 and Supplementary Figs 1 and 2 and Supplementary File 1).
Primers used for qRT-PCR are shown in Supplementary Table 2. VitB6 extraction and measurements of total vitB6 content from fresh and lyophilized tuber samples were done by a yeast bioassay, adapted from a protocol described in [ 9], and a vitB6 auxotrophic strain, ATCC9080.
Our comparison starts with a classic in gel digestion method, which is tested in combination with SDS-containing and SDS-free protein extractions (Supplementary protocol 1 and Supplementary protocol 2).
Second, we would like to offer some observations regarding their data extraction (see Supplementary website material) and pooled analysis.
A total of 403 regions of attachment were shared between both extraction protocols (Supplementary Material, Tables S1 and S2 for complete list along with other chromosomes assayed).
Data were abstracted into standard fields, such as study aims, design, methods, setting and participants (see online supplementary appendix 4 'Data extraction template'), 30 and entered into QSR International's NVivo V.10 software to assist qualitative analysis and synthesis.
The antibodies used in this study are listed in Supplementary Table 2. RNA extraction, reverse transcription, and quantitative reverse transcription-PCR were performed as previously described (Okabe et al, 2009, 2012).
An overview of the variables we plan to extract is presented in the data extraction form (see online supplementary table S2).
A linear external damping coefficient is applied to enable power extraction and a supplementary mass is introduced to tune the floater to the incoming wave conditions.
Most of the analysis in this study was carried out using EZH2 antibody CSTD2C9 which recognises both EZH2p and EZH2α in western blots (Fig. 5a), and gives relatively evenly distributed nuclear foci by immunofluorescence in either primary or adapted cells before extraction (Fig. 6d, Supplementary Fig. 3).
Thus, studies have focused on producing things such as fertilizer and calcareous soil improvement (Ch'ng et al. 2013; Thongpae et al. 1992), animal food (Youburee 2003), and supplementary food from pineapple fiber extraction (Upadhyay et al. 2010; Utama-ang and Tepjaikad 2001).
