One noticeable feature regarding HupBMtb, based upon sequence alignment of HU orthologs from different bacteria, was the presence at its C-terminal end a highly basic extension (Supplementary Figure S1), designated as the C-terminal region (CTR), which prompted us to examine its role.
We also provide evidence that inhibition of ethanolamine driven PE synthesis via the Kennedy pathway by ECT1Δ PCT1Δ double deletion abolishes ethanolamine-mediated lifespan extension (Supplementary Figure S3E).
A similar reduction in rRNA methylation was observed for the snR75mut, relative to the wild-type snoRNA, when the RNA was analysed by primer extension (Supplementary Figure S8).
Addition of various concentrations of PCNA (up to 6.6 nM), at sub-equimolar concentrations (compared to Pol δ), resulted in more efficient extension (Supplementary Fig. 1, lanes 5 8).
We analyzed the extent of the lymph node metastasis at the first operation, which included maximum metastatic tumor size, N stage, and presence of extranodal extension (Supplementary Table S1).
Thermal cycling conditions applied to each assay consisted of an initial Taq activation step at 95°C for 15 min followed by 38 cycles of denaturation (95°C), annealing (range 50.0°C to 64.2°C, see Supplementary Table S1), extension (72°C) and an amplicon-specific fluorescence acquisition reading (range 74°C to 84°C).
For completeness, we have also displayed the cerebello-thalamo-cortical pathway from cerebellar cortex to cerebral cortex by showing slice-by-slice its extension in Supplementary Materials (Supplementary Figure 1).
Fascin 1−/− hindbrain vessels generally displayed fewer filopodia extensions (supplementary material Movies 1 and 2).
Similar argument also negates the role of K11, K29 and K63 ubiquitin residues in the polyubiquitin chain extensions (Supplementary Figure S6A).
Since peaks differ a lot in their extensions (Supplementary Fig. S2), we took a region of 100 nt centred on the summit as the predicted binding region.
A supplementary lateral view during extension can be useful to assess reducibility of atlanto-axial subluxation possibly limited by pannus tissue between the anterior arc of the atlas and dens.
