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Exact(1)
At entry the application of the supplementary exclusion the criteria (list of exclusion criteria) further reduced the number of preterm infants to N=110.
Similar(59)
See online Supplementary Methods for exclusion criteria.
Cell viability was analyzed by Trypan blue dye exclusion (Supplementary Materials and Methods) and cell cycle distribution was assessed by FACS analysis as described previously.
This difference was not due to an effect of the genotype on the number of neurosphere-forming cells or cell viability, as shown by clonal assays (Fig. 3B) and PI exclusion (supplementary material Table S1), respectively.
Nine of these (25%) met the proposed IDSA case definition of PTLDS, while the remaining 27 (75%) had persistent symptoms after treatment of Lyme disease but failed to meet supplementary inclusion or exclusion criteria in the IDSA case definition.
Patients meeting a less stringent definition with a history of physician-documented Lyme disease and current fatigue, musculoskeletal pain or neurocognitive dysfunction but failing to meet supplementary inclusion or exclusion criteria relating to the timing of symptom onset or the presence of minor depression were also included, but are identified and presented separately.
Supplementary table A lists exclusion criteria.
No subjects met this criterion for exclusion (see Supplementary Table 1).
Thus, 10 patients who met all inclusion and exclusion criteria (Supplementary Methods Table T1) were enrolled.
We also determined how our implementation scaled for increasing input sequence lengths and reference exclusion scenarios (Supplementary Fig. S24a).
These data are consistent with the reduction in the percentage of living cells revealed using the Trypan blue exclusion assay (Supplementary Figure 3).
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