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Posthumous collections of Olson's work include A Nation of Nothing but Poetry: Supplementary Poems, edited by George F. Butterick (2000), and Collected Prose, edited by Donald Allen and Benjamin Friedlander (1997).
AS conceived of and designed the study, was in charge of creating the survey, conducted supplementary analyses, edited and revised the text.
A major concern to automated authorship has been ensuring that authorship cannot be 'manipulated' by spurious, short-lived edits (Supplementary Text S1).
Neuronal migration was analysed in Masp1 knockout embryos generated by CRISPR/Cas9 gene editing (Supplementary Fig. 1c,d,g).
In a similar manner, the role of MASP2 in migrating neurons was examined, using Masp2−/− embryos generated by CRISPR/Cas9 gene editing (Supplementary Fig. 1e,f,h).
Thirty-four genes are predicted to be affected by these gains and losses, including two separate olfactory receptor groups, several genes with possible roles in cancers of the prostate, breast and colon, a gene from the HLA-D locus, and two proteins thought to be involved in RNA editing (Supplementary Table 4).
Interestingly, genes with decreased recovery in tdp-1 ok803 tdp-1 ok803 enriched for neuronal-specific genes, many of which are highly edited (Supplementary Table S8, bottom).
Using a compiled list of all nucleotide positions that showed a discrepancy between the RNA and DNA sequence (see Materials and Methods), we hand-annotated 210 regions containing convincing evidence of A-to-I RNA editing (Supplementary Table S4).
Cep-2 differs from its plastomic counterpart by several mutations, including two premature stop codons in chlB, of which one of the two cannot be replaced by neither U-to-C nor C-to-U RNA-editing (supplementary fig. S5, Supplementary Material online).
On the flip side, in the pathways 'Ribosome' and 'RNA polymerase' only 50% and 33% of the identifiable proteins are edited and 0% and 4% of the identifiable proteins are heavily edited, respectively (Supplementary file 1I), demonstrating that some crucial pathways are protected from editing.
N.R. and X.W. provided edits and supplementary text.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com