Information on the primers and probes is shown in Supplementary Table 1.The dye for Reporter 1 was VIC, and the quencher was NFQ; for Reporter 2, the dye was FAM, and the quencher was NFQ.
Over 60% of the cells were PKH67-positivePKH67-positiveincubation with H. pofygyrus vesincubationared with 1.5% wH.n incubated with background dye (Supolygyrusry Fig. 5).
It is evident from Fig. S1 of the Supplementary Material that dye decomposition was enhanced notably with the increase of both Fe2+ and H2O2 concentration at the same ratio.
We also observed TDE of R9-HiLyte nona-arginine peptide labelled with anotheR9-HiLyte nona-argininelementaR9-HiLyte nona-arginine
We expressed wt and BrC6K mutant ZHER2 proteins and fluorescently labeled them with the FITC dye (Supplementary Figure S12).
Further, to improve circulation and prevent uptake by the reticuloendothelial system, nanoparticles were pegylated, which did not impair uptake by cells as shown using nanoparticles loaded with a dye (Supplementary Figure 4A).
Real-time RT-PCR reactions were performed using Brilliant SYBR Green dye (Supplementary Table S 2) with two technical replicates per samples for each of three biological replicates, and data were analyzed with MxP3000 4.01 software (Stratagene).
To examine the in vivo degradation behavior of the PA-RGDS, PA-RGDS that was prelabeled with CM-DiI, a red fluorescent dye (Supplementary Figure 1A), was allowed to self-assemble into a three-dimensional gel.
Therefore, translocation of the selected mutants was also confirmed by following quenching of the DNA-tethered fluorescent dye (Supplementary Figure S6) A complete inability to displace streptavidin, translocate and unwind duplex DNA was observed for the R88A mutant.
The observation that there was rapid uptake of ICG dye into the RPE in the early phase following systemic injection was further corroborated by AO-ICG images of the inner retinal vasculature that we acquired during the first five minutes following dye injection (Supplementary Figure 4).
Four compounds enhanced cell death, as measured by metabolic dye reduction (Supplementary Figure S4).
