Supplementary domains assist in targeting and substrate specificity [ 24], in contrast to most other types of phosphatases, which require interaction with regulatory proteins for proper substrate binding.
> Among the 26 probable proteins, 13 contain only eukaryotic domains, 12 contain only prokaryote-original domains, and the other one has no characteristic domain (supplementary data S7, Supplementary Material online).
In plants, roughly 70% of the domain-containing proteins are single domain (supplementary fig. 4, Supplementary Material online).
We found that in most lineages, RGS is fused to at least one TM domain (supplementary fig. S7, Supplementary Material online) but in apusozoans, amoebozoans, and haptophytes.
We noted that chimeras contain various well-characterized complete protein domains (Supplementary Material), representing most protein domains (69%) of those present in the GENECODE 3C (human) database (Table 1).
GST pull-down results also showed that BARD1 HP1γ interaction is affected by the ubiquitination level of BARD1 BRCT domain (Supplementary Fig. 4d), which is regulated by USP15 (Supplementary Fig. 4e).
Olig1 interacts with Smad3 through the MH2 domain (Supplementary Figure S5A), whereas Smad3 interacts with Olig1 through the bHLH and the C-terminal regions (Supplementary Figure S5B).
Comparisons of Pfam domains (Supplementary Note IXa) identified 17 C. milii domains that are missing in bony vertebrates (Supplementary Table IX.2).
Importantly, this mutation does not impair the overall fold or stability of the PHD1 domain (Supplementary Figure 2).
Previously unreported somatic mutations and deletions of AJUBA were primarily 5′ inactivating events and clustered missense mutations in the functional LIM domain (Supplementary Fig. 4.2).
