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This GI was next to a tRNA and is probably involved in benzoate degradation (supplementary fig. S4, Supplementary Material online).
We observed that simultaneous usage of MG132 and CQ on Flag-ULK1 WT transfected HEK293 cells blocked ULK1 degradation (Supplementary Figure 8c).
Furthermore, high-concentration polyacrylamide electrophoresis of the human frontal cortex RNA failed to reveal evidence of non-specific degradation (Supplementary Figure S1).
We therefore suggest a preliminary model for MEX-3C-mediated mRNA degradation (Supplementary Figure S6).
Treatment with a proteasome inhibitor suggested that the decrease in SARM expression is due to protein degradation (Supplementary Figure S7d).
However, mutation of K231, the major ubiquitination site, did not prevent PERK-mediated SENP3 degradation (Supplementary Figure 3B and C).
In fact, the remaining fragment of ICAM-5 after cleavage was barely seen suggesting degradation (supplementary material Fig. S2).
All statistical analyses were conducted using SPSS 20.0 (SPSS Inc .. Supplementary Figure 1: Degradation of PA-RGDS in vivo.
Pin1 bound efficiently also to N4-ICD, and silencing of Pin1 accelerated N4-ICD's proteasome-dependent degradation (supplementary Fig S5K, middle and lower panels).
In contrast, deletion of the last 376 AAs (constructs D3, D4 and D5) from the C-terminus did not affect Def-mediated p53 degradation (Supplementary information, Figure S3B).
In contrast, treatment with the complex I inhibitor rotenone did not result in outer membrane protein degradation (Supplementary Material, Fig. S1C).
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