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Primers were designed to amplify the ortholog in M6 and a linked sequence in DM1 3 (Supplementary Data 4).
These first-step mutants contained single, non-synonymous mutations to gyrA and no other mutations (e.g. in efflux pumps) linked to ciprofloxacin resistance (Supplementary Data 1).
Notably, we also identified many transcription and nuclear factors whose expression profiles were strongly linked to each cell type (Supplementary Data 1).
Quantity of physical and electrochemical processes was evaluated and linked to the state of specimen providing valuable supplementary data.
Description and links to Supplementary Videos 1 4, Supplementary Data, and Supplementary Software.
We identified 149 unique, high confidence residue cross-links in total (Fig. 2c and Supplementary Data 2).
In total, 12 different mutations in genes and/or cellular processes previously linked to mecillinam resistance were identified in this resistance group (Supplementary Data 2)42.
Spectra representing for each species have been deposited to Proteome Commons database https://proteomecommons.org/ [12] (Link: https://proteomecommons.org/tranche/data-downloader.jsp?h=9FYJgZ%2Bvzxy7TQtjfkLTPclKFDMB4uRYbbSupplementaryy2%2FXhPKzS42hWSVoyxhTRVRMBJsbdataafXq1trhjC7cAAAAAAAAIgw%3D%3D) (Supplementary data online).
The supporting MS data for each cross-link of the purified M1 protein, albumin, and fibrinogen are listed in Supplementary Data 1.
Supplementary information: Supplementary data are available at Bioinformatics online.
Supplementary data (for total mortality data).
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