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qRT-PCR validated the RNA-seq data, confirming that ISRIB completely blocked upregulation of these transcripts (Supplementary Figure S4).
Further, Supplementary data confirm this point by showing that the methylation of TMS1 gene in the U87 and U251 cells was associated with the corecruitment of Dnmt3b, HDAC1 and Dnmt1 on the TMS1 promoter (Supplementary Figure S6).
Alkaline phosphatase enzymatic activity and matrix mineralization were decreased in MT2A-silenced cells compared with control cells (Supplementary data 3B E, respectively), confirming that MT2A influences osteoblastic cell differentiation.
Where available, deletion map data confirmed that these genes were located in centromeric chromosome bins (supplementary table S2, Supplementary Material online).
The data confirms that down-regulation of HNF1α exacerbates hepatic fibrogenesis (Supplementary information, Figure S4).
Our previous data confirmed that rAAV 1/2-based vectors get trapped in the ventricle wall (Supplementary Fig. 1).
Our data confirm that CD99 ligation does not increase NK92 cell cytotoxicity against TC252 or SKNMC cells (Supplementary Figure 2).
Self-report data confirmed that the expectation model (E2(E1(S2)) = ½ × E1(M1) × I) is an accurate representation of the Trustees' average second-order expectations (see Supplementary Figure 1).
Firstly, the new data confirms that anomalous deceleration exists.
Our data confirms that this is the case.
Recent data confirm that this is still the case.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com