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Volatility requires supplementary data about the target system.
The primary data is obtained in the main experiment whereas the secondary data includes all supplementary data about the analyzed subjects.
They were also asked what they called the organizational model (self-identification) and to provide supplementary data about medication administration, patient and work allocation, and scheduling of shifts on the ward (see Additional file 1B).
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All constructs were verified by DNA sequencing (see Supplementary Data 3 for information about oligonucleotide primers, DNA, and amino-acid sequences).
All constructs were verified by DNA sequencing (see Supplementary Data 4 for information about oligonucleotide primers, DNA, and amino-acid sequences).
All constructs were verified by DNA sequencing and termed NCXCL-Aga2pC fusion proteins (see Supplementary Data 5 for information about protein accession numbers, oligonucleotide primers, DNA, and amino-acid sequences).
Mutagenic oligonucleotides were designed to introduce single point mutations at the desired sites and generate 54 hCXCL1 variants (pCT-hCXCL1ALAn-Aga2, NhCXCL1ALAn-Aga2pC; Supplementarytary Data 6 for information about hCXCL1 mutants, oligonucleotide primers, DNA, and amino-acid sequences).
All constructs were verified by DNA sequencing (Macrogen) and termed NFc-CXCLC fusion proteins (see Supplementary Data 1 for information about protein accession numbers, oligonucleotide primers, DNA, and amino-acid sequences).
All constructs were verified by DNA sequencing and termed NCXCL-SAC fusion proteins (see Supplementary Data 2 for information about protein accession numbers, oligonucleotide primers, DNA, and amino-acid sequences).
Besides, we did an analysis of the promoter targets of C/EBPβ in HL-60 cells by ChIP-seq (Supplementary Data 4), and noticed that about 1/3 of JMJD3-upregulated genes were also the direct target genes of C/EBPβ, displaying a significant correlation (p = 4.49 × 10−9, Fig. 6b).
Fasciated ARs (Supplementary Data Fig. S2E) appeared on about 20%% of the explants, with a mean number of 2·08 (±0·4) per explant.
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