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A supplementary confirmation was proposed for these tests because, rigorously speaking, the ANOVA assumption on equal variance is slightly violated.
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We identified 839 additional cases through the drug reimbursement database and used the supplementary questionnaire for confirmation.
In total, we tracked 153 cells from three datasets, with most tracking from the primary dataset and the two others used for confirmation (supplementary material Table S1).
Compounds with ≥25% inhibition in the screen (100, 0.16% hit rate) were cherry picked and confirmed by retesting, with a 73% confirmation rate (Supplementary Table S1).
Proliferation assay (Fig. 1b and Supplementary Fig 1c for confirmation results), cell cycle analysis (Fig. 1c) and apoptosis assay (Fig. 1d) also indicated that the miR-155ko/ko cells have enhanced proliferation defects in low glucose condition.
After confirmation by immunoblotting (Supplementary Figure S6), optimal sense (5'-GCAGUUCCUAUUGUAUAUATT-3') and antisense (5'-UAUAUACAAUAGGAACUGCTT-3') sequences were selected for SPRY4 RNA interference (synthesized by Shanghai GeneCore Biotechnologies Co., Ltd).
The primers used for confirmation are listed in Supplementary Table S1.
Putative de novo CNVs were tested for confirmation using qPCR (see Supplementary Information for more details on the qPCR replication).
As shown above, the final peak assignment was achieved by additional digestions using specific exoglycosidases (results not shown) and by confirmation using MALDI TOF-MS (SuppleMALDI TOF-MSe Supplementary
If possible de novo CNVs were detected, these were tested for confirmation using qPCR (see Supplementary Information for more details on the qPCR replication).
Confirmation of identified phosphorylation sites (Supplementary Table S3).
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