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The electromagnetic parameters of the samples were measured using a vector network analyzer (Agilent 8722ES) in the range of 1-18 GHz after a full two-port calibration (Supplementary Fig. S4 S6).

The cryo-EM map is then corrected for the magnification error, according to the previous microscopic calibration (Supplementary Materials).

2) The cryo-EM map is then corrected for the magnification error, according to the previous microscopic calibration (Supplementary Materials).   3) The cryo-EM reconstruction is placed at the center of a P1 cell with 'crystal' unit cell dimensions at least twice the model diameter.

The TOF spectra were converted to energy spectra using an energy conversion function empirically derived from a set of calibration curves (Supplementary Figs 10 and 11) as described in Supplementary Note 6 and Supplementary Fig. 12.

Eight fossils were used as calibration points (Supplementary Table 2) for the crown node of the specified taxa.

Supplementary Figure 6 Axial calibration of the photometric ratio and axial estimation error caused by small single-mode aberrations.

To dissect sources of differences in molecular age estimates, we ran analyses under three fossil calibration schemes (Supplementary Data 2) and the 105-species phylogenomic tree.

MiR-215-5p MiR-215-5p MiR-215-5p MiR-215-5psexpressionstudy was performed by use of calevelsinn CRCve (Supplementary Fig. S7A) and absolute quantificellsn.

The % dicamba removal was calculated with the help of the calibration curve (Supplementary material 1).

The concentration of the excess Dox was determined by the absorbance measurement at 480 nm using a calibration curve (Supplementary Figure S4).

Chips were analysed using a PBS-II SELDI-TOF (Ciphergen Biosystems) with conditions and calibration described in Supplementary methods.

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