These analyses supported the findings from the isolation-migration analysis with only minor differences among data sets (Additional file 1 Supplementary Information, bracketed parameters are collared flycatcher Ne, pied flycatcher Ne, ancestral Ne, migration rate, migration rate from pied to collared flycatcher and migration rate from collared to pied flycatcher, e.g. the full model being (ABCDE)).
Numerous OCB peptides mapped to clusters of related IgG-VH sequences that were represented in >1 band on the IEF gels (see Fig 4, gray brackets; Supplementary Table S3), indicating the presence of related IgG in spatially separated OCB.
Users can enter structures in dot-bracket format (Supplementary Material Sections S1.3, S1.6 and S1.7).
The abnormal appearance of the lateral stripes in mutant larvae can be attributed to defects in patterning of regeneration and metamorphic melanophores (supplementary material Fig. S13F,G, brackets), suggesting abnormal migration or differentiation of this lineage.
Foxa2 expression, which marks the distal (future anterior) region of the primitive streak (Bra+/+, white bracket, Fig. 7D) and is also expressed in extraembryonic endoderm (Burtscher and Lickert, 2009) is clearly present in Bra-null embryos (Bra−/−, white brackets, Fig. 7D; supplementary material Fig. S7A), and is seemingly upregulated in the mid/proximal primitive streak.
The most prominent phenotype for each gene is highlighted in bold text and the percent of cells showing this phenotype is shown in brackets (see Figure 1, Supplementary. Figure 2 for examples of each phenotype).
The range of the mean size HpaII digested zLMO DNA is shifted compared to the mean size of the control DNA, but we did not observe the appearance of the low molecular weight band observed for MspI digestion, compare lanes 3 and 4; black bracket (wild type); red bracket (zLMO); ethidium gel in Supplementary S6).
Surveys with over 200 subjects from a discrete population or in-depth interviews with more than 50 participants fitted into this bracket, for example (see Supplementary Materials).
B6129 ΔCS mice were produced by an exchange mutation in FasL gene sequence, which replaces four residues bracketing two enzymatic cleavage sites (Supplementary Figure 1C).
For clarity, when referring to cell strains each strain code is represented by an abbreviated syndrome prefix with the strain code in brackets, e.g., SS GM09812) for Seckel Syndrome cells (see Supplementary Table 1).
A full-length Tu-CAS (tetur10g01570) homologue was retrieved in T. evansi by sequencing PCR products bracketing the CAS gene with neighboring genes (Supplementary file 4).
