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The multiple supplementary bands observed in the blot would be explained by the renowned propensity of scFv to form dimeric and higher-order aggregates.
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In order to further determine if KLF15 binds to the promoter sequence, super-shift assay was performed using anti-KLF15 mAb but no supplementary band was detected.
These include closed reduction and fracture fixation using inter-fragmentary screws or cannulated screws with or without supplementary tension band wiring through the screw.
It seems very unlikely the PeSL mini-circles are merely bound to the exterior surface of the virion or are leftover recombinational intermediates from the host cytoplasm, which contaminate the stock because phage stocks treated with DNase I prior to nucleic acid extraction still produced the same inverse-PCR bands (supplementary fig. S2, Supplementary Material online).
Analysis of Cbt and GAF in polytene chromosomes confirmed their co-localization in particular bands (Supplementary Fig S3A and B).
Age, as a covariate, had a significant relationship to GFS scores in all bands (supplementary material S8).
In Podocarpus elongatus, the PCR using 5SgR/18SR and 5SgF/26SF yielded one prominent plus several minor bands (Supplementary Figure S2).
The presence of several isoforms from the 2D gels was also apparent by 1D WB analysis in which the expression of GAPDH was observed as a band corresponding to the molecular mass of full-length GAPDH together with a number of lower molecular mass bands (Supplementary Figure 1).
LC-MS/MS analysis of the proteins in the AP1 band revealed the identity of proteins present in this band (Supplementary Table S1).
Peptides from three other CDXG kinases were also detected in the same gel band (Supplementary file 1A).
The specificity of antibodies was previously tested by immunoblotting and showed only one specific band (Supplementary Figure 1).
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