Further details of the statistical methods are available in the supplementary attachment (available online only).
A breakdown of the reasons for dropping out from follow-up is shown in the supplementary attachment (available online only).
The same phenomenon can also be observed in a time-lapse video of cell attachment (Supplementary material).
The no-load transport velocity was measured independently either before or after the force-velocity measurements and there was no significant difference between velocities measured before or after microsphere attachment (Supplementary Figure 4).
These results are in contrast to knockdown of talin by 40% that produced a corresponding decrease in HSB-2 T cell attachment (Supplementary Figure 3), suggesting a key role for talin, and not α-actinin-1, in this step.
In contrast, in MCF-7/MR cells, ABCG2-EGFP was targeted exclusively to the membrane of EVs and cell-cell attachment zones (Supplementary Figure S2 D F).
After all candidate nodes attached to the substrate and formed FAs, further extension of the spreading area were achieved by allowing the attached FAs to move on the x-y plane in radial orientation against the center of attachment area (Supplementary Fig. S1).
Subsequent to the establishment of integrin KD in hMSC, we investigated for changes in their cell attachment, spreading (Supplementary Figures 2 and 4b) and migration on collagen I.
Treated cells showed very effective attachment, which revealed that combined treatments did not significantly affect cell adhesion (Supplementary Figure 3).
Removal of one myosin type from the scaffold was achieved by introducing a photocleavable element in selected myosin-attachment DNA linkers (Supplementary file 1).
In Crim1 flox/flox; Pdgfb-iCreER mutant pups, the attachment of desmin+ (supplementary material Fig. S5) or NG2+ pericytes (data not shown) to VECs at the angiogenic front was not compromised, although smooth muscle actin (SMA) showed mildly reduced labeling around arteries (supplementary material Fig. S5E-H).
