Secondary objectives: To determine: the prevalence of maternal postpartum depression at 6 8 weeks postpartum; if the current, Province wide, self-report based screening for postpartum depression is sufficient to adequately detect AD among postpartum women, or if supplementary anxiety specific screening is needed; and the course of mood symptoms from pregnancy to 6-months postpartum.
Secondary outcomes were: perceived physical and mental health status, psychological well-being and state-trait anxiety (Supplementary data are available in Age and Ageing online, file 5).
Supplementary tests for anxiety-like behaviour and locomotion were carried out on the same mouse strains in order to rule out potential confounds with the evaluation of behavioural despair after the treatment with T2.
Self-report visual analogue scale questionnaires administered before (baseline) and 4.5 h after (follow-up) amino acid drink ingestion measured various aspects of mood, aggression, and physical symptoms thought to be associated with ATD including nausea, dizziness, irritability and anxiety (Supplementary material).
Separately, we also assessed the effects of a bolus administration of 3,5-diiodo-L-thyronine (T2) in mice in the tail suspension test, a common test of depressive-like behaviour, and in supplementary paradigms for anxiety and locomotion, such as dark/light and novel cage tests.
In addition, no differences were found between the Mbd5 +/GT and the Mbd5 +/+ mice in the plus maze, a more sensitive test to evaluate anxiety levels (Supplementary Fig S4C), further supporting the conclusion of normal anxiety in the mutant mice.
To exclude the possibility that the mPFC regulates anxiety relief independent of pain state, we optogenetically activated PL excitatory neurons unilaterally in naive mice, and no obvious changes in anxiety-like behaviours were observed (Supplementary Figs 9a d), indicating that the relief of anxiety-like behaviours in the CFA mice was pain-related.
To order the genes according to their significance with regard to anxiety disorders, a supplementary analysis was performed to examine whether significant trends in associations could be detected across individual genes.
At the individual level, determinants of receipt of minimally adequate care included age, having a family physician, a supplementary insurance coverage, a comorbid anxiety disorder and the severity of depression.
No significant differences between Ts and wild-types were found (Supplementary Material, Fig. S1A) in anxiety-related behavior.
The MRI experience caused mild discomfort (0.44) and anxiety (0.44) (see online appendix, supplementary table S3).
