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However, many other MODs now have some type of community annotation portal for researchers to submit annotations to the curators or upload supplementary annotations, including SGD (http://wiki.yeastgenome.org ), WormBase (http://wiki.wormbase.org ), TAIR (www.arabidopsis.org/doc/submit/functional_annotation/123 ), and MGI (www.informatics.jax.org/submit.shtml ).shtml
To identify all metabolic genes and map them into KEGG pathway, supplementary annotations were created against the KEGG database and the UniProtKB database using BLASTX (Match No. ≥ 50 aa, Identity ≥ 25.0%, E-value ≤ 1.0E-20 1.0E-20e use the InterProScanddatabase to search for the functional motif (E-value ≤ 1.0E-4).
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Regardless of the accuracy of the reference annotation, the novel FP transcripts have higher expression estimates than the FP transcripts present in the annotation (Supplementary Note).
The repeat-masked ATLCOD1A then underwent whole-genome structural alignment and re-ordering using the three-spined stickleback genome as reference followed by projection annotation Supplementary Note 17 of [ 3].
For supplementary tables annotations using PANTHER database [ 28] (http://www.pantherdb.org/) were included.
We evaluated the classification method on a gold standard derived from the PubMed Supplementary Concept annotations, achieving a micro-average F1-score of 85.9% for primary subtypes.
Any supplementary functional annotation information is included if available.
Only 5 genes away from this cluster, another small cluster containing PKS, NRPS, P450s and an ABC transporter was identified, suggesting these two could be part of a larger cluster (see Additional file 3: Supplementary CDC annotation list).
The atlas is publicly available and includes the confidence parameters, the SNP annotation, and the gene annotations (supplementary table S3, Supplementary Material online).
However, Panther and GO annotations (supplementary file S9, Supplementary Material online) suggest that protease-encoding genes are under comparatively high selection pressure.
Two complete prophages, unique to the clade II genomes, are clearly identified in figure 2. We assigned new open reading frames (ORFs) and updated the annotations (supplementary tables S2 a and b, Supplementary Material online) as described in the Materials and Methods.
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