Introduction of T48I mutation in the resulting strain WRG23 was visualized by supplementary addition of 40 µg 5-Bromo-4-chloro-3-indolyl-phosphate toluidine salt (BCIP) per plate and deep blue colonies, indicating overexpression of PhoN, were selected.
For this, RA FLSs were cultured in the presence of sFasL for 24 hours either with medium alone or with the supplementary addition of TGF-β (10 ng/ml), since TGF-β has been described to strongly induce VEGF165 [ 31, 32].
The bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive of supplementary substrate additions or exogenous manipulations.
Fungal strains belonging to the genera Alternaria, Cladosporium, Epicoccum, Fusarium, Mucor, Penicillium, Pestalotiopsis, and Trichoderma were cultured directly from visibly fouled concrete structures and used individually and in a mix to inoculate mortar tiles varying in cement composition, supplementary cementitious material additions, water-to-cement ratio, and surface roughness.
D.M.A. applicants are required to submit supplementary materials in addition to the items listed above.
Induced Ca2+ overflow was observed in the cytoplasm of myocytes upon ionomycin addition (Supplementary Figure S4b).
Secondary fuel means a fuel fired through a burner other than the primary fuel burner that provides supplementary heat in addition to the heat provided by the primary fuel.
In addition, supplementary to the standard paradigm of uniform traffic volumes between randomly interacting node pairs, more realistic gravity traffic patterns are used to simulate the flows in the network.
In addition, supplementary meteorological masts were installed in advance at Gochang and Wangdeung-do in order to enhance the estimation of the long-term wind potential for the demo-farm.
The encapsulated clay minerals had a large capacity for binding and capturing DNA from solution, and retained DNA in the clay-DNA hydrogel that formed after electrolyte addition (Supplementary Figure 4 28.
(see Supplementary Material) In addition, the inclusion of SOC also leads to an indirect gap of 1.64 eV between the highest VB state and lowest CB state, which is 0.04 eV lower than the direct band gap.
