Finally, we suggest the authors to provide a supplemental step-by-step guide to replicate the results that they describe in the paper.
On average, we collected information on 85% of individuals and 84% of households at each step (Supplemental Tables 1 and 2): 73% of households missed no more than one interview; 6.5% missed five or six interviews.
Notably, the version of pGG eC carrying An2 improved ent-kaurene yield threefold relative to the original AtCPS-derived construct (Fig. 7) and was found to offer higher yields with several different ent-kaurene synthases, indicating that the production of ent-CPP had been a limiting step (Supplemental Fig. 4).
Indeed, whatever the concentration used, the venom PLA2 did not induce any plasma membrane damage, as no release of LDH was observed upon the preincubation step (see supplemental Fig S1B) or upon plating for 5 h (see Fig. 1F).
With the application of an alkaline extraction stage, separation of acetic acid from deacetylated feedstock may require only a supplemental dewatering or filtration step.
In brief, the model is a physics-based hydrology model coupled with an individual-based entomology model that is run at a spatial resolution of 10 m with a 1-hr time step (see Supplemental Material, Figure S1).
[ 4] where two beeps (7 ms duration) were played at different time delays between 36 204 ms (12 ms steps; see Supplemental Information and Figure S1 available online).
In a first step, we removed "supplemental oxygen", not relevant in intubated patients.
After quality filtering, the remaining 31,396,183 pairs were assembled with Trans-ABySS using k-mer values from 23 to 47 in steps of 4 (Supplemental Table S1).
We cut out a piece of silicon rubber (McMaster-Carr, 87315K63) of the same dimensions as the coverslips that will be used (25 mm round) (see Supplemental Material S1 for more detailed illustrated step-by-step assembly and usage protocols).
We summarize the training steps in the following paragraphs, and we have also included a step-by-step facilitator guide in the Supplemental Material.
