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H2O2 standard (Sigma) was used to generate a standard curve to calibrate this reaction (Supplemental Figure S6).
For all in-tube enzyme assays described in the supplemental information, the reaction mix was incubated with shaking at 37°C in 20 mM sodium acetate, pH 5.2.
ETD MS/MS spectra were acquired using: supplemental activation enabled, reaction time = 100 msec, reagent automatic gain control target = 1 E5 ion counts, full AGC target = 3 E4, isolation width of 3 m/ z.
Folates are required for nucleotide synthesis and S-adenosylmethionine (SAM) –dependent methylation reactions (Supplemental Figure 1).
Breedings were performed between heterozygous eGFP and homozygous NOD.CB17- Prkdc scid genotyped by polymerase chain reaction (PCR) analysis (Supplemental Results; Supplemental Fig. 1 A).
Supplemental cross-linking (postcuring) reaction of PGS prepolymer was done in vacuum oven at 100°C for additional 48 hours.
This result suggests that enhanced dopaminergic function during imagination subsequently increases estimations of future hedonic reaction (see Supplemental Results for additional ANOVA results).
Number of peptide cleavages observed for synthetic H3 fragment using different ETD reaction times vs. supplemental activation with CID or HCD.
In the House, however, the reaction to the supplemental has been more resistant than receptive.
For a complete list of primer sequences and reaction conditions, see Supplemental Table 1.
See Supplemental Information for primer details and reaction conditions.
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