The Supplemental experimental procedures (Supplemental data S14) include lists of antibodies and primers used in all experiments and supplemental protocols.
Tables S1 S3, Figures S1 S5, and Supplemental Protocols 1 and 2 Table S4.
To enable facile adoption of the GMAP array for large-scale screen deconvolution, we have also provided a series of supplemental protocols and software tools to help extract data from functional screens using commercially available gene modulation libraries.
DNA was isolated from 3-day-old females (n = 10) using the QIAGEN DNeasy Blood and Tissue Kit according to the supplemental insect protocol.
All chemicals except for substrate solutions (Item 5 on page 5 of the supplemental ELSA protocol document) were ordered from Sigma-Aldrich.
However, this new unconventional technique might not suffer from the same non-completion rate once it is established that it can work and becomes part of the regular workflow, instead of being presented as a supplemental research protocol.
Supplemental experimental protocols, complete data tables of RNA-Seq, TMT-MuDPIT, and GO annotation analyses, and supplemental figures supporting the data presented in the main text.
Respiratory settings were modified according to the PEEP/F io2 table of the ARDS Net protocol (supplemental data, Supplemental Digital Content 1, http://links.lww.com/CCM/B420).lww.com/CCM/B420
Fully detailed methods and protocols are available in the online supplement, Supplemental Data S1.
One example, Dynamis, is a peer-to-peer supplemental unemployment insurance protocol that uses the policy holders' social capital to replace underwriters.
