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A Perl script (tmavalid.pl) for validating TMA data exchange files is included as a supplemental attachment with this publication.
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It is hoped that TMA files will soon be available to the public as supplemental attachments to journal submissions.
In particular, the rabbit stifle had no visible supplemental meniscal root attachments as noted in the human knee, (Ellman et al. 2014; LaPrade et al. 2014; Johannsen et al. 2012) although this observation may be due to the considerably smaller scale of the New Zealand white rabbit stifle.
Attach lower attachment assembly.
Attach supplemental documents.
Initial results, shown in Supplemental Figure S1, demonstrated preferential attachment and expansion of Accutase-dissociated hESC on Cultispher-S.
An Optovue RTVue (Optovue Inc., Fremont, CA, USA) and a supplemental cornea-anterior module (CAM) attachment were used for the epithelial and corneal pachymetry scans.
Supplemental fibers of the medial anterior root attachment, lateral anterior root attachment, and medial posterior root attachment were too small to differentiate from ACL or PCL fibers.
Briefly, cells were plated onto specialty plates (Costar ultra low attachment clusters), containing the culture medium lacking supplemental LIF.
WPMY-Vec and WPMY1-AR cells were plated in equal numbers in androgen-free medium for attachment then transferred to fresh medium with or without supplemental 10 nM DHT for 72 hrs.
The lesser height of BF-fixed nuclei in this experiment compared to others (Fig. 2 and supplemental online material S 1) is probably due to the prolonged attachment on poly- L-lysinated slides overnight.
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