ELISA plates were measured at 650 nm, or 450 nm after supplemental addition of 1 N HCl, with a microplate reader (Benchmark Plus, Bio-Rad, Hercules, CA).
Our results indicated that a supplemental addition of the collagen peptide-containing drink to the usual meal facilitated pressure ulcer healing, since the collagen peptide-containing drink led to more changes in the DESIGN-R scores.
Initially, C. aspera consumed simultaneously both EcO157 and aerobic bacteria carried over from wastewater, in contrast to Platyophyra sp. requiring a supplemental addition of 5% CGM as nutrients to initiate consumption of EcO157 and aerobic bacteria.
Recombinant CCL-1 injected intrathecally (i.t). to naive mice induced allodynia, which was prevented by the supplemental addition of N-methyl-𝒟-aspartate (NMDA) receptor antagonist, MK-801.
It would be relevant in this respect to grow C. thermocellum with a suitable co-culture partner strain on high cellulose loadings to see whether overflow metabolism occurs and whether cross-feeding of vitamins can eliminate the need for supplemental addition of vitamins during the course of the fermentation.
Our results strongly indicate that supplemental nutrient additions are required to increase freshwater productivity in nutrient-limited streams of central Idaho.
Litter of Pinus edulis, Juniperus monosperma and Populus deltoides were exposed to ambient and attenuated sunlight, with and without supplemental water additions, at a riparian forest site along the Middle Rio Grande (New Mexico, USA).
In addition, we found evidence that supplemental pollen addition reduces the mass of individual achenes, which suggests that female reproductive output is constrained by resource availability.
However, this represented a 34% decrease in ethanol yield when compared to the WSF without supplemental glucose addition (Figure 1C) although, due to the addition of the glucose, a final concentration of ~72 g/l ethanol could be achieved.
